JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ346935.1
Database:	nt	Go to original record
Description:	Nauclea diderrichii chloroplast DNA for trnT-L-F region, specimen voucher Razafimandimbison 297 (LBR, MO, P, TAN)
Taxon:	Nauclea diderrichii
Original size:	1725 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	752	1321	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttagatg cattagtaga gagatctttc catcgaaaat tcagaaagaa
251   tgaagtgaag gataaacgta tatacatacg tattgaatac tatatcaaat
301   gattaatgac gacccgactg aatctgtatt tttatatatg aaaaacagaa
351   gaattggtgt gaatagattc cacattgaag aaagaatcga atattcattg
401   atcaaatgat tcactccata gtctgataga tctttgcaag aactgattaa
451   tcggacgaga ataaagatag agtcccattc tacatgtcaa tgtcggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details