Match details
Record ID:
M86847.1
Database:
nt
Go to original record
Description:
Synthetic Plasmid pWB305 constructed to function as a substrate for measurements of the fidelity of PCR. Carries a lacZ gene flanked by two selectable markers, kanamycin resistance, and supF.
Taxon:
synthetic construct
Original size:
8018 bp
View other 1 match for this record
View other 59426 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
613
827
+
215 bp
1 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201 gccaggctca aggcg
View other 57490 matches with an identical amplicon
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
3.06e-09
primerB
100.0
21 bp
0
0
215
195
3.06e-09
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.