JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430939.1
Database:	nt	Go to original record
Description:	Tetrachondra patagonica chloroplast tRNA-Leu gene for transfer RNA-Leu
Taxon:	Tetrachondra patagonica
Original size:	1151 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	309	858	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggttcagaa
151   agcaaaaaag ggataggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgga atctttccat cgaaacttca gaaaggatga
251   aggataaatt tatatattga atactatatc aaattattaa tgaagaccct
301   aatctttatt tttgtatatg aaaaatggaa gaattggtgt gaattgattc
351   cacattgaag aaagaatgga atatttattg atcaaatcat tcactccata
401   gtctgataga tcttttaaag aactgattaa tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa tactggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details