JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430978.1
Database:	nt	Go to original record
Description:	Stylidium bulbiferum chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Stylidium bulbiferum
Original size:	1337 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	353	935	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcccatttt ccgaaaacaa gagttcaaaa
151   aacaaaaatc aagaaggata ggtgcagaga ctcgacggaa gctgttctaa
201   cgaatggagt tgattgtctt gcgttggtat agaatacttt ttttgaaaag
251   tccgaaaaga tgaaggataa acccatatat atatgggtat gtgtacgaaa
301   atagtatatc aaaggattcg acgcgagttt tgatcggcct tttttagata
351   aaaaggggaa gaattgttgt aaatccattt tatattgaag aatgaatcta
401   ctattcattg atcaaatcat tcaccccggg tctgagaaat cttttgaaga
451   cctgataatc ggacaagaat agaataaaga tagagtccca ttctacatgt
501   caatagtggc aacgatgaaa tttatagtaa gaggaaaatc cgtcgatttt
551   cgaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details