JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430973.1
Database:	nt	Go to original record
Description:	Scaevola sp. Lundberg 55 chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Scaevola sp. Lundberg 55
Original size:	1219 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	261	824	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaacccagg aattaataaa
101   aaggggcaat cctgagccaa atctcgtttt cataaaacaa ctaaagtcag
151   aaagcgcaaa taaaaaggat aggtgcagag actcgatgga agctgttcta
201   acgaatggag tgaattgcct tggttggtag aggaatcctt ctattgaaat
251   ttcagaaaag accaagtata acctatatta tgcataatag aatataaata
301   atatatgcct aatagaattt caatttatag aatttcaatt tcaatataga
351   agaagtgttg tgaatcgatt cattccatat cggaatattc atcgatcaaa
401   tcattcactc cataatctga tagatctttt gaagaagtga ttaatcggac
451   gagaataaag atagagccct cttttacatg tctataccgg caataatgaa
501   atttatagga aaaggaaaat ccgttgattt aaaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details