JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430938.1
Database:	nt	Go to original record
Description:	Polypremum procumbens chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Polypremum procumbens
Original size:	1255 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	335	894	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcara raaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttagaa
151   agcgaaaaaa gggataggtg cagagactca atggaagcta ttctaacaaa
201   tggagttgac tgtggaatcc ttccatcgaa acttccgaaa ggatgaagga
251   twaacgcata tattgaatac tatatcaaat gattaatgaa gacccgaatc
301   tttttttttt ttatatgatt tttttatatg aaaaatggaa gaattggtgt
351   gaattgattc cacattgaag aaagaatgga atatttatta atcaaatcat
401   tcactccata gtctgataga tctttaaaag atctgattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa tactggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details