JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430928.1
Database:	nt	Go to original record
Description:	Phryma leptostachya chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Phryma leptostachya
Original size:	1180 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	259	819	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aacgggcaat cctgagccaa atcctctttt ctcaaaagaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gagtaatctt tccatcgaaa cttcagaaag
251   gataaaggat aaacgtatct atcgaatact atatcaaata attaatgata
301   gcccgaatct gtatctgtat tttttaatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatcg aatattcatt catcaaatca
401   ttcactccat agtccgatag gtcttttaaa gaaatgatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details