JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ984182.1
Database:	nt	Go to original record
Description:	Eriogonum umbellatum tRNA-Leu gene, partial sequence; plastid.
Taxon:	Eriogonum umbellatum
Original size:	614 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttaattg gttgagcctt agtatggaaa
51    cctactaagt gagaactttc aaaatcagag aaaccctgga attaaaaaaa
101   atgggcaatc ctgagccaac tcctgctttc caaactttcc aaaagggaga
151   agaaataaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttgactg tcttgcgttg ttataagaat tcttccacag aaactcaaaa
251   aaggatgaag aataaaccta tatgcatacg tactttcgta ctttaattta
301   aattactaaa aatagaaaat aaaaacgaat aatgaattct gtattttttt
351   aatatgcaaa aaaagatgaa aaatagaaga attgtcgtga agttattcca
401   agtttaagaa agaatcgaat attcgtttat tttctattaa aaagattaaa
451   acatttactc cacagtctga tagatctttt gaagaactga tcaatcggat
501   gagaatgaag atagagtccc attctacgtg tcaataccga caacaatgca
551   atttagagta agaggaaaat ccgtcgactt tataaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details