JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ984179.1
Database:	nt	Go to original record
Description:	Arabis nuttallii tRNA-Leu gene, partial sequence; plastid.
Taxon:	Arabis nuttallii
Original size:	579 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	579	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctggtttacg tgaacaaacc ggagtttaga
151   aagcgagaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt cactaccttg tgttgataaa ggaatccttc gatcaaaact
251   tcaaatcaaa aaggttgaag gagaaaaacc tatattgtct aaatataggt
301   aacacaaaac gatctcaaaa atgacgacct gaatctcgat ttctattttt
351   ttataaacaa aatcgaaatg atgtgaatca attcgaagtt taagaaataa
401   tattcattga tcaaatgatt ctcttcatag tctgatagat ccgtggtgga
451   acttattaat cggccgagaa taaagataga gtcccatttt acatgtcaat
501   actgacaaca atgaaattta tagtaagatg aaaatccgtt gacttgttaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details