JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ984173.1
Database:	nt	Go to original record
Description:	Phlox hoodii tRNA-Leu gene, partial sequence; plastid.
Taxon:	Phlox hoodii
Original size:	621 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	621	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ttttttcgaa aacaaacaaa
151   gattcggaaa gtaaaaataa gaatcaaaag gaaggatagg tgcagagact
201   caacggaagc tgttctaaca aatggatttt actgcgttgg tcaaggaatc
251   ttttcatcga aacttaagaa aggatgaaag aaaaacatat atccatatgc
301   atacgtactg aaatactata ttaatttatt aaactatatc cattttttaa
351   taagtaccaa aaccagtatt cgtatgtttt ctatatataa aaaaaaagaa
401   agaagtgttc tgaatagatt ccacaaagaa taaagaattg aatgttcgtt
451   gatcaaataa attaatccat agtctgatag atcttttgaa gaacttatta
501   atcggacaag aataaagata gagtcccatt ctacatgtca ataccgacag
551   caatgaaatt tatagtaaga ggaaaatccg tcgacttcag aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details