JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430926.1
Database:	nt	Go to original record
Description:	Paulownia tomentosa chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Paulownia tomentosa
Original size:	1302 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	344	908	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaacccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttytaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgacg gataaacgta tctattgaat actatatcaa atgattaatg
301   atggcccgaa tctgtatctg tattttttaa tatgaaaaaa tgaaagaatt
351   ggtgtgaatt gattccacat tgaagaaaaa gttgaatatt cattcatcaa
401   atcattcact ccatagtccg atagatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaagga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details