JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ695425.1
Database:	nt	Go to original record
Description:	Spermacoce remota isolate H10 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Spermacoce remota
Original size:	1837 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	802	1386	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccactgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctatttt ccgcaaacaa aaaaggggtt
151   cagaaagtga aaaaggggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttggatgcgt tagtcgataa gtctttccag gaaaaattcc
251   ttaagtagtt aaggataaag cgaaggataa acgtatatac atacgtagtg
301   aatagcatat taaatgagta ctgacagccc aaactaatct ctattttttc
351   tatgaaaaaa gaaagaattg ttgttaatag attctatgtt taagaaagaa
401   tcgaatattc attgatcaaa tgattcactc catagtctga tagatctttt
451   cacgaactga ttaatcagac gagaataaag atagagtccc attttacatg
501   tcaattccgg caacaatgaa atttatagtt aagaggaaaa tccgtcgact
551   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details