JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ695419.1
Database:	nt	Go to original record
Description:	Pentodon pentandrus isolate H38 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Pentodon pentandrus
Original size:	1728 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	750	1342	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aaaataaagg
151   ttcagaaagt gaaaaagggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggatgc gttagtcgag aagtctttcc aggaaaaatt
251   tataaagtat aagtatttaa ggatgaagtg aaggataaac atatatacat
301   acgtagtgaa tagtatatta aatgagtaat gacagcccaa atgaatctct
351   attttttcta tgaaaagcta aagaattgtt gttaacagat tccacgttta
401   agaaagaatc gaatattcat tgatcaaatg attcactcca tagtctgata
451   gatcttttca cgaatggatt aatcagacaa gaataaagat agagtcccat
501   tttacatgtc aatgccggca acaatgaaat ttatagttaa gaggaaaatc
551   cgtcgacttt taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details