JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430917.1
Database:	nt	Go to original record
Description:	Sanango sp. Bremer 3352 chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Sanango sp. Bremer 3352
Original size:	1281 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	322	882	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaagg gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacatatct attgaatact atatcaaatg attaatgatg
301   gcccgaatct gtatctgtat ttttgtatat gaaaaaagga agaattggtg
351   tgaattgatt ccacatcgaa gaaagaatcg aatattcatt catcaaatca
401   ttcactccat agtctgataa atattttaaa gaacttatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca ataccggcaa caatgaaact
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details