JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ695405.1
Database:	nt	Go to original record
Description:	Coprosma pumila isolate B43 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Coprosma pumila
Original size:	1321 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	350	935	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccactgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgcgatagt cgagaagtct ttccatcaaa aattcataaa
251   gtataagtat taaggatgaa gtgaaggata aacgtatata catacgtagt
301   gaatagtata ttaaatgagt aatgatagcc cgaatgaatc tttatttttt
351   catagaaaaa ataaagaatt gttgttaata gattccacgt ttagaaaaga
401   atcgaatatt cattgatcaa atgattcact ccatagtctg atagatcctt
451   ttacgaactg attaatcgga cgagaataaa gatagagtcc cattctacat
501   gtcaatgccg gcaacaatga aatttatagt taagaggaaa atccgtcgac
551   ttttaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details