JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ150606.1
Database:	nt	Go to original record
Description:	Ixora vieillardii voucher Mouly et al. 322 (P) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ixora vieillardii
Original size:	1608 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	745	1310	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggga
51    aacccactaa gtgataactt tcaaattcag agaaaccctg gaattaataa
101   aaaggggcaa tcctgagcca aatcctgttt tccgaaacca aaggtagaga
151   aagtgaaaag gggataggtg cagagactca acggaagttg ttctaacaaa
201   tggagttggc tgcgttagta gagaaatctt tccatcgaaa attccgaaag
251   gataaagtga aagataaagg tatatacgta ttgaatacta tatcaaatga
301   ttaatgacga ctcgactgaa tctgtatttt ttatatgaaa acggaagaat
351   tggtgtgaat agattccaca ttaaagaaag aatcgaatat tcattgatta
401   aatgattcac tccatagtct gatagatctt ttcaagaatt gattaatcgg
451   acaagaataa agatagagcc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details