JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ150599.1
Database:	nt	Go to original record
Description:	Ixora sp. Malcomber et al. 2980 voucher Malcomber et al. 2980 (MO) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ixora sp. Malcomber et al. 2980
Original size:	1603 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	747	1306	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccaaaaccaa aggtacagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagttgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaaggt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctttttatat gaaaacggaa gaattggtgt
351   gaatagattc cacattaaag aaagaatcga atattcattg attaaatgat
401   tcactccata gtctgataga tcttttcaag aattgattaa tcggacgaga
451   ataaagatag agccccgttc tacatgtcaa tgtcggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details