JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ150581.1
Database:	nt	Go to original record
Description:	Ixora scheffleri voucher Luke 9162 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ixora scheffleri
Original size:	1608 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	747	1311	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggtacagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagttgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaaggt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa gggaagaatt
351   ggtgtgaata gattccacat taaagaaaga atcgaatatt cattgattaa
401   atgattcact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagccc cgttctacat gccaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details