JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ150547.1
Database:	nt	Go to original record
Description:	Ixora casei voucher Mouly & Florence 348 (P) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ixora casei
Original size:	1556 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	758	1322	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggtacagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagatgt tctaacaaat
201   ggagttggct accttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaaggt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa gggaagaatt
351   ggtgtgaata gattccacat taaagaaaga atcgaatatt cattgattaa
401   atgattaact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagccc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details