JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430931.1
Database:	nt	Go to original record
Description:	Plantago argentea chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Plantago argentea
Original size:	1294 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	336	917	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactagg tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctcaaaataa aggttcagaa
151   agcgagaaaa agggataggt gcagagactc gatggaagct gttctaaaac
201   aaatggagtt gactgcgttg gtagataaat tttttcatcg aaacttcaga
251   aaggatgaag gataaacgta tctattctat tgaatactaa aatatctaaa
301   aaaatgaatg acggcccgag tcggtatctg tatttttttt tagatgaaag
351   atggaagaat tggtgtgaat tgattccaca ttcaagaatg aatcgaatat
401   tcattcatca aatcacccca ctccatagtc tgatagttct ttttaaaaag
451   aacttatatt aattggacga gaataaagat agagtcccgt tctacatgtc
501   aataccgaca gcaatgaaat ttatagtaat aggaaaatcc gtcgactttt
551   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details