JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430946.1
Database:	nt	Go to original record
Description:	Montinia caryophyllacea chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Montinia caryophyllacea
Original size:	1168 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	217	772	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt acgcaaacaa aggttcagaa
151   accgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtaa aggagttttt ccatcaaaac ttccgaaaga
251   atgaaggata aatatataca tatgtattga atatatcaaa tgattaatga
301   caatccgaat ttgtattttt tcaatgaaaa atgtaagaat tggtgagaat
351   cgattccaca ttgaagaaag aatcgaatat tcattgatca aatcattcac
401   tccatagttc gataaatctt ttgaagaact gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatatc gacaacaatg aaatttatag
501   taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details