JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430945.1
Database:	nt	Go to original record
Description:	Kaliphora madagascariensis chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Kaliphora madagascariensis
Original size:	1318 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	358	919	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattcaaaaa
101   atgggcaatc ctgagccaaa tcctgtttta cgaaaacaaa caaaggttta
151   gaaaacgaaa caaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactacatta gtcaaggagt ttttccatca aaacttccta
251   aagaatgaag gagaaatata tacatatgta ttgaatacta tatcaaatga
301   ttaatgacga tccgaatctg tattttttct atgaaaaatg taagaattgg
351   tggaatcgat tccacattga agaaagaatc gaatattcat tgatccaatc
401   attcactcca tagtctgata aatcttttga agaactgatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aatatcgaca acaatgaaat
501   ttatagtaag aggaaaatcc gttggcttaa aaggtcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details