JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430924.1
Database:	nt	Go to original record
Description:	Lindenbergia sp. Thulin 8079 chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Lindenbergia sp. Thulin 8079
Original size:	1279 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	342	893	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   gggggcaatc ctgagccaaa tcctgttttc taaaaacaaa gacaaaaata
151   aaggataggt gcagagactc aacggaagct gttctaacaa atggagttga
201   ttgcgccggt agaggaatct ttccatcgaa acttcggaaa ggatgaagga
251   taaacgtatc tattgaatac tatatcaaat tattaatgat ggcccgaatc
301   tgtatctgta ttttttaata tgaaaaatgg aaaagttagt gtgaattgat
351   tccatattga agaaagaatc gaatattcat tcatcaaatc attcactcca
401   cagtccgata gatcttttaa agaattgatt aatcggatga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details