JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430919.1
Database:	nt	Go to original record
Description:	Lamium album chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Lamium album
Original size:	1197 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	330	865	+	536 bp

Amplicon:

536 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa acccgggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ataggtgcag agactcaatg gaagctgttc taacaaatgg agttgactgc
201   gttggtagag gaatcttttc tacggaaact tcagaaagga taaccgtatc
251   tatcgaatac tatatcaaat gattaatgat ggcccgaatc cgtatttttt
301   catatgaaaa atagaagaat tggtgtgaat tgcttctata ttgaagacaa
351   aatagaatat tcattcatca aagcattcac tccatagtcc gatagatctt
401   ttaaagaact gattaatcgg acgagaataa agagagagtc ccgttctaca
451   tgtcaatact ggcaacaatg caatttatag taagaggaaa atccgtcgac
501   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	536	517	2.41e-08

Match details