JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430914.1
Database:	nt	Go to original record
Description:	Jacaranda mimosifolia chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Jacaranda mimosifolia
Original size:	1303 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	346	906	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagat gttctaacaa
201   atggagttga ctgcgttggt agaggaatct ttccatcgaa acttcagaaa
251   ggatgaagga taaacgtatc tattgaatac tatatcaaat gattaatgat
301   agcccgaatc tgtatctgta ttttttatat gaaaaataga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatcg catattcatt catcaaatca
401   ttcactccat agtccgatag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca atcccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details