JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430930.1
Database:	nt	Go to original record
Description:	Globularia cordifolia chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Globularia cordifolia
Original size:	1255 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	325	874	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcagaacaa agattcaaaa
151   agcgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaacc
201   ggagttgact gcgttggttg atgaatcttt tcatcgaaac ttcagaaagg
251   atgaaggata gacgtatcta tttaatacta tatcaaataa ttaatgatag
301   atcaaatctc tatctgtatt tttttatagg aaaaatgaaa ggattgattc
351   tacattgaag aaagaatcga atattcattc atcaaatcat tcactccata
401   gtctgataga tcttttaaag aacttattaa tcggacgaga ataaagatag
451   agtcccattc tacatgtcaa taccgacaac aatgaaattt atagtaagag
501   gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details