JRC GMO-Amplicons | EURL GMFF

Record ID:	FM207123.1
Database:	nt	Go to original record
Description:	Crossopteryx febrifuga chloroplast trnT-trnL IGS, tRNA-Leu gene, trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher UPS Bremer 3097
Taxon:	Crossopteryx febrifuga
Original size:	1716 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	795	1362	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattactaaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt tcatcgaaaa ttccgaaagg
251   ataaagtgaa agataaacgt atatacgtat tgaatactat ataaaatcaa
301   atgattaatg acgactcgac tgaatccgtt ttttatatga aaacggaaga
351   attggtgtga atagattcca cattgaagaa agaatcgaat attcattgat
401   taaatgattc actccatagt ctgatagatc ttttcaagaa ttgattaatc
451   ggacgagaat aaagatagag tcccgttcta catgtcaatg tcggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details