JRC GMO-Amplicons | EURL GMFF

Record ID:	FM207118.1
Database:	nt	Go to original record
Description:	Augusta rivalis chloroplast tRNA-Thr gene (partial), trnT-trnL IGS, tRNA-Leu gene, trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher ETSU McDowell 5017
Taxon:	Augusta rivalis
Original size:	1748 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	790	1352	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcattagtag ataaatcttt ccatctaaaa ttccgaaaga
251   aggataaacg tatatatacg tattgaatac tatatcaaat caaatgatta
301   atgacgactc gaatgaatct gtattttttt tatgaaaacg gaagaattgg
351   tgtgaataga ttccacattg aagaaagaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgat agatcttttc aagaattgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatgtcggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details