Match details

Record ID: FM207140.1
Database: nt Go to original record
Description: Scyphiphora hydrophyllacea chloroplast trnT-trnL IGS, tRNA-Leu gene, trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher S K Bremer et al. 99
Taxon: Scyphiphora hydrophyllacea
Original size: 1707 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 803 1368 + 566 bp

Amplicon: 566 bp
1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttagct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaacgt atatacgtat tgaatactat atcaaatgac
301   gactcggctg aatctgtatt ttttatatga aaatatgaaa acggaagaat
351   tggtgtgaat agattgcaca ttgaagagag aatcgaatat tcattaatta
401   aatgattcac tccatagtct gatagatctt ttcaagaatt gattaatcgg
451   atgagaataa agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc 

View other 1 match with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.55e-08
primerB 100.0 20 bp 0 0 566 547 2.55e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.