JRC GMO-Amplicons | EURL GMFF

Record ID:	FM207130.1
Database:	nt	Go to original record
Description:	Nematostylis anthophylla chloroplast trnT-trnL IGS, tRNA-Leu gene, trnL-trnF IGS and tRNA-Phe gene (partial), specimen voucher UPS Lantz 160
Taxon:	Nematostylis anthophylla
Original size:	1576 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	685	1243	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccaccaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggtgaaaaa
151   aggataggtg cagagactca acgggagctg ttctaacaaa tggagttggc
201   tgcgttagta gagaaatctt tccatctaaa attacgaaag gataaagtga
251   aggatagacg tatatatacg tattgaatac tatatcaaat gattaatgag
301   gactcgacta aatttgtatt ttttatatga aaaagggaag aattggtgtg
351   aatagattcc acattgaaga aagaatcgaa tattcattga tcaaatgatt
401   cactccatag tctgatagat cttttcaaga attgattaat cggacgagaa
451   taaagataga gtcccattct acatgtcaat gttggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details