JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430970.1
Database:	nt	Go to original record
Description:	Campanula elatines chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Campanula elatines
Original size:	1352 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	361	946	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aatgaataaa
101   aatgggcaat cctgagccaa atccggtttt ctgacaataa caaaaggttg
151   agaaagcgaa aatctaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gggttgattg tgttgcgttg atagagaaat tcttttatag
251   aaacttccga aaggttgaag tataagccta tactatatat atagttaaga
301   tataggtact gaaatcctat gaaatatcaa aagattaatg acgcctcgaa
351   tccgtatttt ttctatgaaa actggaagaa ttggtgtgta atatattccc
401   tattcaagaa agaatagaca aatcagtcac tccccagtct gagagatctt
451   ttgaagaatt gattaatcgg accagaataa agatagagtc ccattctaca
501   tgtcaatacc ggcaacaatg aaatttatag taataggaaa atccgtcgat
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details