Match details
| Record ID: |
GQ497217.1 |
| Database: |
nt |
Go to original record |
| Description: |
Glycine max transgenic GMO cassette genomic sequence. |
| Taxon: |
Glycine max |
| Original size: |
3210 bp |
View other 25 matches for this record
| Method: |
QL-CON-00-005 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
|
| Description: | Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005). |
| Target: | pat-CaMV T-35S |
View other 212 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
1438 |
1647 |
+ |
210 bp |
|
1 atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51 gctcctccaa ggccagttag gccagttacc cagatctgag tcgacctgca
101 ggcatgcccg ctgaaatcac cagtctctct ctacaaatct atctctctct
151 ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
201 gtttcgctca
View other 140 matches with an identical amplicon
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
2.98e-09 |
| primerB |
100.0 |
23 bp |
0 |
0 |
210 |
188 |
2.61e-10 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
