JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430941.1
Database:	nt	Go to original record
Description:	Byblis liniflora chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Byblis liniflora
Original size:	1241 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	291	852	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaacaaa
101   gatgggcaat cctgagccaa atcctacaaa ggtccagaaa gcgaaaaggg
151   ataggatagg tgcagagact caatggaagc tgttctaaca aatggagttg
201   gctgcagtgg cagtggtaaa ggaatctttc catcgaaact tcagaaagga
251   tgaaggataa actgaattga atactacatc aatagattaa tgatgcacca
301   agtctctatc tttatttttt tttataggaa aaatggacga attgatgtga
351   attgattcca cattgaagaa agaatcgaat ctaatattca ttcagaaaag
401   cattcactcc atagtctgat agatctttta aaaaattgct taatcggacg
451   agaataaaga tagagtccat tctacatgcc aataccggca acaatgaaat
501   ttctagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details