JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430929.1
Database:	nt	Go to original record
Description:	Antirrhinum majus chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Antirrhinum majus
Original size:	1258 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	338	898	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   agcgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgtgttggta gaggaatctt tccatcaaaa cttcagaaaa
251   gatgaaggat aaatctatct attgaatact atatcaaatt attaatgatg
301   gcccgaatct gtatctgtat ttttctatat taaaaatgga agaattggtg
351   tgaattgatt ccacattgaa taaagaatgg aatattcatt cattaaatca
401   ttcactccat agtctggtag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details