JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430923.1
Database:	nt	Go to original record
Description:	Cyclocheilon somaliense chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Cyclocheilon somaliense
Original size:	1263 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	320	870	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaaggat aggtgcagag actcaacgga agctgttcta
201   acaaatggag ttaattgcac tggtagagga atttttccac cgaaacttca
251   gaaaggatga aggataaacg tatcatttga atactatatc aaatgattaa
301   tgatggcccg aatctgtatt tttaaagatg aaaaatggaa gaattggtgt
351   gaattgattc catattgaaa aaagaatatt cctcaaatca gtcattccac
401   agtctgatag gtcttttaaa gaactgattc atcggacgag aataaagata
451   gagtcccatt ctacatgtca ataccggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details