JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430912.1
Database:	nt	Go to original record
Description:	Acanthus longifolius chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Acanthus longifolius
Original size:	1241 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	323	862	+	540 bp

Amplicon:

540 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aacgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aagaaaaaag gataggtgca gagactcaat ggaagctgtt ctaataaatg
201   gagttgactg cattagtaga gaaatctttt catcgaaact tcaaaggata
251   aacgtatcta ttgaatacta tatcaaatga ttagataacc cgtatccata
301   ttttttatat gaaaatggaa gaattggtgt gaattgattc cacattgacc
351   aaagaatgga atattcattc atcaaatcat tcactccata gtccgataga
401   tcttttaaag aacagatcaa tcggacgaga ataaagatag agtcctattc
451   tacatgccaa gaccggcaac aatgaaattt ctagtaaaag gaaaatccgt
501   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	540	521	2.43e-08

Match details