JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ430907.1
Database:	nt	Go to original record
Description:	Alstonia scholaris chloroplast tRNA-Leu gene for transfer RNA-Leu and partial tRNA-Phe gene for transfer RNA-Phe
Taxon:	Alstonia scholaris
Original size:	1339 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	393	946	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcaac ggaagctgtt cttacaaatg
201   gagttggccg cgttggtaga gaaatctttc catcgaaaat tcagaagata
251   aacgtatata catacgtatt gaatactata tcaaatgatt aatgccgacc
301   cgaatgaatc tgtatttttt ctatgaaaaa tggaggaatt ggtgtgattc
351   gattccacat tgaagaaaga atcgaatatt cattgatcaa atgattcact
401   ccatagtctg aagatctttt caagaactga ttaatcggac gagaataaag
451   atagagtccc gttctacatg tcaatgccgg caacaatgaa atttatagta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details