JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ395402.1
Database:	nt	Go to original record
Description:	Ficus carica cultivar Khadhri tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Ficus carica
Original size:	607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	8	597	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg aattgcggcg gatctacgaa
51    cgaggattga acgatccaag gataccttta aattagagaa accctggaat
101   taaaaatggg caatcctgag ccaaatccgg ttttctgaaa acaaacaagg
151   gttcagaagg cgataataaa aaaggatagg tgcagagact caatggaagc
201   tgttctaaca aatggagttg ggctgcggtg cgttatgtaa aggaatcact
251   ccacgaaagg atgaagaata aacctatata cgtatacgta ctgaaatagt
301   atcttcaaat gattaatgac aacccaaatc cgtatttctt ttaattttca
351   tgaaaaatta aagaattatt gtaaatcaat tattaagttg aaaaaagaat
401   taaatattca ttaatcaaat catttatctc catcaaaatc tgatagatct
451   tttgaagaat ggattaatcg gatcggggaa aaagggtatg agtcccactt
501   ttatcatgtc aatttcgaca acaactaaat tttttgtaaa ggagaaaatt
551   ccctgaccaa tattctgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details