JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ395401.1
Database:	nt	Go to original record
Description:	Ficus carica cultivar Khadhouri tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Ficus carica
Original size:	594 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	7	592	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg aatttgaatt acgggatgcc
51    cgaactgcat tgattgacca agtgataact ttaattagag aaaccctgga
101   attaaaaatg ggcaatcctg agccaaatcc ggttttctga aaacaaacaa
151   gggttcagaa ggcgataata aaaaaggata ggtgcagaga ctcaatggaa
201   gctgttctaa caaatggagg ttggctgcgg tgcgttatgt aaaggaatca
251   ctccacgaaa ggatgaagaa taaacctata tacgtatacg tactgaaata
301   gtatcttcaa atgattaatg acaacccaaa tccgtatttc ttttaatttt
351   catgaaaaat taaagaatta ttgtaaatca attattaagt tgaaaaaaga
401   attaaatatt cattaatcaa atcatttatc tccatcaaaa tctgatagat
451   cttttaagaa tggattaatc ggtcgatgaa taaaagagtc ccattttaca
501   tgtcaaatcg caacaataaa ttatgtatag tagaatcccg agccaattgt
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details