JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ395397.1
Database:	nt	Go to original record
Description:	Ficus carica cultivar Gaa Zir tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Ficus carica
Original size:	588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	586	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg aatgatagaa tgggcgttgg
51    gttggaacta ccagtgataa ctttcaaatt cagagaaacc ctggaattaa
101   aaatgggcaa tcctgagcca aatccggttt tctgaaaaca aacaagggtt
151   cagaaggcga taataaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggaggttggc tgcggtgcgt tagtaaagga atcactccag
251   aaaggatgaa gaataaacct atatacgtat acgtactgaa atagtatctt
301   caaatgatta atgacaaccc aaatccgtat ttcttttaat tttcatgaaa
351   aattaaagaa ttattgtaaa tcaattatta agttgaaaaa agaattaaat
401   attcattaat caaatcattt actccatcaa aatctgatag atcttttgaa
451   gaatggatta atcggtcgtg ttttggtagg agtcccattt ttcattgtcc
501   aattcggcac aattgaactt ttattgtaaa aggaaattcc ctttcggaat
551   atttggctga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details