JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ395388.1
Database:	nt	Go to original record
Description:	Ficus carica cultivar Kahli 1 tRNA-Leu (trnL) gene, intron.
Taxon:	Ficus carica
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	6	589	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg aattgaatcg ggttatggac
51    gcattgtagg acatcccagt tgatacttca attagagaaa ccctggaatt
101   aaaaatggga aatcctgagc caaatccggt tttctgaaaa caaacaaggg
151   ttcagaaggc gataataaaa aaggataggt gcagagactc aatggaagct
201   gttctaacaa atggagttgg ctgcggtgcg ttagtaaagg aatcactcca
251   gaaaggatga agaataaacc tatatacgta tacgtactga aatagtatct
301   tcaaatgatt aatgacaacc caaatccgta tttcttttaa ttttcatgaa
351   aaattaaaga attattgtaa atcaattatt aagttgaaaa aagaattaaa
401   tattcattaa tcaaatcatt tactccatca aaatctgata gatcttttaa
451   gaatgattaa tcggcgagat aagagaggtc ccatttacat gtcaaatcgc
501   aacaatgaat atgttataag tccctcttct attttggaat ccgtcgactt
551   taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details