JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801164.1
Database:	nt	Go to original record
Description:	Cercis gigantea voucher Herendeen 1-V-2003-10 (US) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Cercis gigantea (nom. inval.)
Original size:	1067 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaga
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctac
201   caaatggagt tgacggcatt tcgtttcgtt agtaaagtaa tccttccatc
251   aaaactccat aaaagaaagg atcaaagata aacgtatata tacgtatacc
301   taccgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   tttaatattt atatgagaaa tgaaagatgt aaatcgattc aaagttgagg
401   aaaaagtcga atattcattc atcaaatcat tcactccatc atagtccgat
451   agatcttttg aagaactgat taatcggaca cgaataaaga tagagtccca
501   ttctacatgt caataccgac aacaatgaaa tttatagtaa gaggaaatcc
551   gtcgacttta gaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details