JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801158.1
Database:	nt	Go to original record
Description:	Adenolobus pechuelii voucher Oliver et al. 6527 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Adenolobus pechuelii
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcagt
151   tcagaaagtg agaataaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgacg ggatttcgtt tcgttagtaa agtaatcctt
251   ccatcgaaac tacagaaaag aaaggatcaa agataaacgt atatatacgt
301   atacgtaacg aaatactatc tcaactaaat taatgacgac gccgaatctc
351   tatttttgaa tattgatatg agaaatgaaa gatgtaaatg gattcaaagt
401   tgaggaaaaa gtcgaatatt cattcatcaa atcattcact ccatcataat
451   ccgatatatc tttcgaagaa ctgattaatc ggacaagaat aaagatagag
501   tcccattcta catgtcaata ctgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details