JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801157.1
Database:	nt	Go to original record
Description:	Adenolobus garipensis voucher Leistuer et al. 246 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Adenolobus garipensis
Original size:	780 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	605	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcagt
151   tcagaaagtg agaataaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgacg ggatttcgtt tcgttagtaa agtaatcctt
251   ccatcgaaac tacagaaaag aaaggatcaa agataaaata aacgtatata
301   tacgtatacg taacgaaata ctatctcaac taaattaatg acgacgccga
351   atctctattt ttgaatattt atatgagaaa tgaaagatgt aaatggattc
401   aaagttgagg aaaaagtcga atattcattc atcaaatcat tcactccatc
451   ataatccgat agatctttcg aagattaatc ggacaagaat aaagaataaa
501   gatagagtcc cattctacat gtcaatactg acaacaatga aatttatagt
551   aagaggaaaa tccgtcgact ttagaaatcg tgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details