JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801150.1
Database:	nt	Go to original record
Description:	Phanera flexuosa voucher Lewis 1866 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	none
Original size:	1086 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaaa agagtttaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcgtt actaaagtag taatccttcc
251   atggaaactc cagaaaagaa aggatcaaag ataaacgtat atatacatat
301   acgtaccgaa atactatctc aactaaatta atgacgacgc cgaatctcta
351   tttattcata tttataatga aagatgtaaa tggattcaaa gttgaggaaa
401   aagtcgaata ttcattcatc aaatcattca ctccatcata atccgataga
451   tcttttgaag aactgattaa tcggacaaga ataaagatag agtcccattc
501   tacatgtcaa tacggacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details