JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801145.1
Database:	nt	Go to original record
Description:	Tylosema humifusa voucher Carter 690( K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Tylosema humifusa
Original size:	1064 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	587	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcgtt agtaaagtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaggata aacgtatata tacgtatcga
301   aatactatct caactaaatt aatgacgacg ccgaatctct atttttgaat
351   atttctataa gaaatgaaag atgtaaatgg attcaaagtt gaggaaaaag
401   tcgaatattc attcatcaaa tcattcactc catcataatc cgatagatct
451   tttgaggaac tgattaatcg gacaagaata aagatagagt cccattctac
501   atgtcaatac tgacaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details