JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801144.1
Database:	nt	Go to original record
Description:	Phanera ornata voucher Larsen 45006 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Phanera ornata
Original size:	1084 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaaa agagttcaaa
151   aagttagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacggtatt tcgtttcgtt agtaaagtag taatccttcc
251   atcgaaactc cagaaaagaa aggatcaaag ataaacgtat atatacatat
301   acgtaccgaa atactatctc aactaaatga atgacgacgc cgaatctcta
351   ttcattaata tttataatga aagatgtaaa tggattcaaa gttgattgag
401   gaaaaagtcg aatattcatt catcaaatca ttcactccat cataatctga
451   tagatctttt gaagaactga ttaatcggac aagaataaag atagagtccc
501   attctacatg tcaatactga caacaatgaa atttatagta agaggaaaat
551   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details