JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801143.1
Database:	nt	Go to original record
Description:	Phanera bidentata voucher Stone 12643 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Phanera bidentata
Original size:	1024 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	606	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaaa agagttcaaa
151   aagtgagaca aaaagtgaga ataaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaaatgga gttgacggga tttcgtttcg ttagtaaagt
251   agtaatcctt ccatcgaaac tccagaaaag aaaggatcaa agataaacgt
301   atatatacat atatgtaccg aaagactatc tcaactaaat gaatgacggc
351   gccgaatctc tattcattaa tatttataat gaaagatgta aatggattca
401   aagttgattg aggaaaaagt cgaatattca ttcatcaaat cattcactcc
451   atcataatcc gatagatctt ttgaagaact gattaatcgg acaagaataa
501   agatagagtc ccattctaca tgtcaatact gacaacaatg aaatttatag
551   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details