JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801138.1
Database:	nt	Go to original record
Description:	Lasiobema penicilliloba voucher Larsen & al. 31900 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Phanera penicilliloba
Original size:	1070 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaataat
101   gggcaatcct gagccaaatc ctgtttttcg aaaacaccaa aaagagttca
151   aaaagtgaga ataaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgacggga tttcgtttcg ttagtaaagt agtaatcctt
251   ccatcgaaac tccagaaaag aaaggatcaa agataaacgt atatatacat
301   atacgtaccg aaatactatc tcaactaaat gaatgacgac gccgaatctc
351   tattcattaa tatttataat gaaagatgta aatggattca aagttgattg
401   aggaaaaagt cgaatattca ttcatcaaat cattcactcc atcataatcc
451   gatcgatctt ttgaagaact gattaatcgg acaagaataa agatagagtc
501   ccattctaca tgtcaatact gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details