JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801134.1
Database:	nt	Go to original record
Description:	Bauhinia mollis voucher Hughes 2402 (K) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia mollis
Original size:	1088 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	604	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agaattcaaa
151   aagttagaat aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaaagtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tacgtatacg
301   taccgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   cttgaatatt tatataagaa atgaaagatg taaatcgatt caaatcaaag
401   ttgaggaaag agtcgaatat tcattcatca aatcattcac tccatcataa
451   tccgatacga tagatctttt gaagaaccga ttaatcggac aagaataaag
501   atagagtccc attctacatg tcaatgctga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
601   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details